What is principle of UV Visible Spectroscopy?

What is principle of UV Visible Spectroscopy?

The Principle of UV-Visible Spectroscopy is based on the absorption of ultraviolet light or visible light by chemical compounds, which results in the production of distinct spectra. Spectroscopy is based on the interaction between light and matter.

What are the application of UV Visible Spectroscopy?

UV/Vis spectroscopy is used in analytical chemistry for the quantitative determination of different analytes, such as transition metal ions, highly conjugated organic compounds, and biological macromolecules.

What is UV visible Spectrophotometer PDF?

Ultraviolet- Visible Spectroscopy. Ultraviolet and visible (UV-Vis) absorption spectroscopy is the measurement of the. attenuation of a beam of light after it passes through a sample or after reflection from. a sample surface. The visible spectrum ranges from 400 nm to about 800 nm.

How do you perform a UV-VIS spectroscopy?

1. Fill the cuvette with the sample.
2. Place the cuvette in the spectrometer in the correct direction.
3. Cover the cuvette to prevent any ambient light.
4. Collect an absorbance spectrum by allowing the instrument to scan through different wavelengths and collect the absorbance.

Which detectors are used in ultraviolet UV Visible Spectroscopy?

Detectors. The photomultiplier tube is a commonly used detector in UV-Vis spectroscopy. It consists of a photoemissive cathode (a cathode which emits electrons when struck by photons of radiation), several dynodes (which emit several electrons for each electron striking them) and an anode.

What is the source used for visible radiation in UV-visible spectrophotometer?

Two sources are commonly used in UV-Visible spectrophotometers: The deuterium arc lamp. Provides a good intensity continuum in the UV region and useful intensity in the visible region. A half-life of the lamp (the time required for the intensity to fall to half of its initial value) is approximately 1,000 hours.

What is bandwidth in spectroscopy?

The spectral bandwidth is defined as the band width of light at one-half the peak maximum, as shown in Fig. The spectral bandwidth of a spectrophotometer is related to the physical slit-width and optical dispersion of the monochromator system.

What is stray light in UV?

Stray light is defined as any light that reaches the detector which is outside the spectral region isolated by the monochromator. High stray light frequently leads to deviations from the Beer-Lambert Law, with subsequent inaccuracies in sample photometric values.