What are the steps in bacterial identification?
When identifying bacteria in the laboratory, the following characteristics are used: Gram staining, shape, presence of a capsule, bonding tendency, motility, respiration, growth medium, and whether it is intra- or extracellular.
How do you identify the identity of bacteria?
Bacteria are identified routinely by morphological and biochemical tests, supplemented as needed by specialized tests such as serotyping and antibiotic inhibition patterns. Newer molecular techniques permit species to be identified by their genetic sequences, sometimes directly from the clinical specimen.
What are the methods of identification of microorganisms?
Among the techniques we use are: DNA sequencing – to identify bacteria, moulds and yeasts. Riboprinter analysis – for bacterial identification and characterisation. Repeat–based polymerase chain reaction – for assessing the similarity of microorganisms.
What is the initial step in morphology and identification of bacteria?
An initial step in identifying a bacterial species is determining whether the cells in question are Gram- positive or Gram-negative. The Gram stain is one of the most important and widely used tools in the identification of unknown bacteria.
What are the principles of bacteria identification?
Identification of bacteria (including mycobacteria) is based on growth characteristics (such as the time required for growth to appear or the atmosphere in which growth occurs), colony and microscopic morphology, and biochemical, physiologic, and, in some instances, antigenic or nucleotide sequence characteristics.
Why is bacterial identification important?
In microbial ecology, the identification of microorganisms helps us characterize biodiversity. Because the clinical samples will most likely contain many microorganisms, both normal flora and pathogens, it is important to isolate the pathogen in a pure culture using various types of selective and differential media.
What is the standard resource for identifying bacteria?
Answer b. Bergey’s Manual of Determinative Bacteriology is the standard resource for identifying bacteria.