What are Daudi cells?
The Daudi cell line is composed of B lymphoblasts isolated from the peripheral blood of a 16-year-old, Black, male, Burkitt’s Lymphoma patient in 1967. These well-characterized B lymphoblasts have been extensively used in the study of leukemogenesis. The line is a suitable transfection host, as well.
How do you develop cell lines?
The simplest way to create a new cell line is to modify an existing one, a common strategy when an established line already comes close to meeting the requirements. Cells optimized to grow particular viruses or maximize recombinant protein production often come from such modifications.
Can you buy cell lines?
You can also buy cell lines from well-known life science brands such as Sigma or Thermo Fisher. [5] These companies typically have smaller collections, but they are usually partnered with big repositories as well.
What is Daudi tumor?
Daudi is a Burkitt lymphoma cell line of East African origin expressing an abnormal ß2m transcript with a start codon mutation (ATG to ATC) in- compatible with translation initiation (28).
How do cell lines become immortal?
Immortalized Cell lines are cells that have been modified in a manner such that they can be cultured indefinitely [6]. This is achieved by either isolating and culturing tumors cells and culturing them, or by introduction of an immortalizing gene.
How many cell lines exist?
3,600 cell lines
Cell line popularity can be estimated by the numerous publications using cell lines and American Type Culture Collection (ATCC) Cell Biology Collection which consists of over 3,600 cell lines from over 150 different species.
What type of T cells are Jurkats?
Jurkat cells are an immortalized line of human T lymphocyte cells that are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV.