What does glutathione S-transferase do?
Glutathione S-transferases (GSTs) are ubiquitous enzymes that are encoded by a large gene family, and they contribute to the detoxification of endogenous or xenobiotic compounds and oxidative stress metabolism in plants.
How is glutathione S-transferase activity calculated?
The GST activity is determined by measuring the rate of produced conjugation between reduced glutathione and CDNB, which is proportional to the increase in absorbance at 340nm over time (ΔOD340nm/min).
What is CDNB assay?
The Glutathione S-Transferase (GST) Assay Kit utilizes 1-Chloro-2,4-dinitrobenzene (CDNB) which is suitable for the broadest range of GST isozymes. Upon conjugation of the thiol group of glutathione to the CDNB substrate, there is an increase in the absorbance at 340 nm.
Why are drugs conjugated to glutathione?
The mercapturic acid pathway (Figure 1) involves the conjugation of the tripeptide, GSH, to xenobiotics (including drugs) to render them more hydrophilic and facilitate their elimination.
Why does increasing enzyme concentration speed up the reaction?
Increasing Substrate Concentration increases the rate of reaction. This is because more substrate molecules will be colliding with enzyme molecules, so more product will be formed.
What are glutathione S-transferases?
Glutathione- S -transferases (GSTs) are a family of Phase II detoxification enzymes that catalyse the conjugation of glutathione (GSH) to a wide variety of endogenous and exogenous electrophilic compounds. GSTs are divided into two distinct super-family members: the membrane-bound microsomal and cytosolic family members.
What are glutathione catalysts (GSTs)?
Specifically, GSTs catalyse the conjugation of glutathione (GSH) to a wide variety of endogenous and exogenous electrophilic compounds ( Figure 1 ). Glutathione conjugation is the first step in the mercapturic acid pathway that leads to the elimination of toxic compounds. GSTs have evolved with GSH, and are abundant throughout most life forms.
What is the pathophysiology of glutathione conjugation?
Glutathione conjugation is the first step in the mercapturic acid pathway that leads to the elimination of toxic compounds. GSTs have evolved with GSH, and are abundant throughout most life forms. GSTs are divided into two distinct super-family members: the membrane-bound microsomal and cytosolic family members.