What does the QIAshredder column do?
The QIAshredder is a unique biopolymer shredding system in a microcentrifuge spin-column format. It homogenizes cell or tissue lysates to reduce viscosity. Homogenization shears the high-molecular weight genomic DNA and other high-molecular-weight cellular components to create a homogenous lysate.
How does RNeasy work?
Total RNA binds to the RNeasy membrane, contaminants are efficiently washed away, and high-quality RNA is eluted in RNase-free water. Using the mechanical disruption method, yeast cells are lysed and homogenized by high-speed agitation in the TissueLyser or other bead mill in the presence of glass beads and Buffer RLT.
Why use RNase free water?
The presence of nucleases such as DNase and RNase in water can degrade precious molecular samples and even ruin experiments. To prevent DNA and RNA sample loss, it is essential that highly pure, nuclease-free water be used in applications such as PCR, cDNA synthesis, nucleic acid purification, sequencing, and cloning.
Is RNA cleanup necessary?
Best! Its good to do DNase treatment especially for further downstream applications despite the good 260/280 values. It may not change the results much but it improves the quality of your RNA which gives you more confidence in your results.
Does RNase-Free Water expire?
Expiration: 10 x 2 mL Nuclease Free Water has a shelf life of up to 2 years from analysis when stored in a sealed container in ambient conditions.
What is the qiashredder?
The QIAshredder is a unique biopolymer shredding system in a microcentrifuge spin-column format. It homogenizes cell or tissue lysates to reduce viscosity. Homogenization shears the high-molecular weight genomic DNA and other high-molecular-weight cellular components to create a homogenous lysate.
What is qiashredder™ spin column?
QIAshredder™ spin columns are designed for simple and rapid homogenization of cell and tissue lysates. Format: Mini spin columns. Sample source: Animal or human cell or tissue lysates, plant cell or tissue lysates, white blood cell lysates. Sample size: Up to 700 µl cell lysate. Homogenization time: 2 minutes.
What is the qiashredder column score in Bioz stars?
Qiashredder Columns, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS – scores, article reviews, protocol conditions and more
Can I use a qiashredder spin column with rneasy MIDI?
We only offer QIAshredder spin columns for use with the RNeasy Mini Kit. When working with RNeasy Midi/Maxi, we recommend using a rotor-stator, or mortar and pestle with needle and syringe homogenization.