How do you Analyse HLA data?

How do you Analyse HLA data?

The high resolution typing method that provides the most accurate and complete identification of HLA genotypes is sequencing-based typing (SBT). For each sample being tested, SBT defines the exact nucleotide sequence of the coding regions of both alleles at a given HLA locus.

What is SBT HLA typing?

Sequence-based typing (SBT) involves PCR amplification of specific coding regions of HLA genes and sequencing of the amplicons [3, 4]. The prevalence of ambiguities in HLA typing relates to the nature of polymorphisms which exists in the sequence of the major histocompatibility complex (MHC) Class I and Class II genes.

Is HLA the same as MHC?

The human MHC is also called the HLA (human leukocyte antigen) complex (often just the HLA).

What is PCR SSO?

Polymerase chain reaction sequence-specific oligonucleotide (PCR-SSO) typing combines the ability to define the finest of HLA specificities, by analysis of the corresponding DNA sequences, with the possibility of study large populations of normal and affected individuals.

What is HLA sequencing?

DNA sequencing is a powerful technique for identifying allelic variation within the human leukocyte antigen (HLA) genes. Sanger sequencing of amplified DNA from each HLA gene from a preparation containing one or two alleles yields a sequence that is used to identify the alleles by comparison with a reference database.

How do HLA genes work?

The human leukocyte antigen (HLA) system (the major histocompatibility complex [MHC] in humans) is an important part of the immune system and is controlled by genes located on chromosome 6. It encodes cell surface molecules specialized to present antigenic peptides to the T-cell receptor (TCR) on T cells.

What is PCR SSOP?

The combination of PCR technology and hybridization with sequence-specific oligonucleotide probes was first applied to HLA class II typing because of the limitations of DR serology and of the better knowledge of allelic polymorphism at DR/DQ loci. …

How does HLA-A* 0261 differ from HLA* 020601?

A new allele, HLA-A* 0261, differs from HLA-A* 020601 by a single-nucleotide substitution at codon 57 (CCG–>GCG) resulting in an amino acid change from Pro to Ala.

What is the DNA sequence of HLA-A11?

“DNA sequence of HLA-A11: remarkable homology with HLA-A3 allows identification of residues involved in epitopes recognized by antibodies and T cells.” Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 26-365 (ALLELE A*11:01). “Molecular analysis of the serologically defined HLA-Aw19 antigens.

How many HLA alleles were identified at the 17th HLA and Immunogenetics workshop?

HLA-A, -B, -C, -DPB1, -DQB1 and -DRB1 were genotyped using a next-generation sequencing method presented at the 17th International HLA and Immunogenetics Workshop. In total, we identified 201 HLA alleles, including 35 HLA-A, 57 HLA-B, 28 HLA-C, 24 HLA-DPB1, 21 HLA-DQB1 and 36 HLA-DRB1 alleles.

What is the HLA-A*24 gene mutation?

“An intronic mutation responsible for a low level of expression of an HLA-A*24 allele.” Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ALLELE A*24:02). “A nucleotide insertion in exon 4 is responsible for the absence of expression of an HLA-A*01 allele.”